A Decoy Library Uncovers U-Box E3 Ubiquitin Ligases That Regulate Flowering Time in Arabidopsis
| Autor: | Jing Hong, Wei Liu, Joshua M. Gendron, Ann Feke |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences biology Transgene food and beverages Protein degradation biology.organism_classification 01 natural sciences 03 medical and health sciences Ubiquitin Arabidopsis Genetic redundancy biology.protein Decoy Organism 030304 developmental biology 010606 plant biology & botany Genetic screen |
| Zdroj: | Genetics. 215:699-712 |
| ISSN: | 1943-2631 |
| DOI: | 10.1534/genetics.120.303199 |
| Popis: | The ubiquitin proteasome system plays an essential role in the regulation of flowering time, a precisely timed developmental transition necessary for plant fitness. However, the full impact... Targeted degradation of proteins is mediated by E3 ubiquitin ligases and is important for the execution of many biological processes. Redundancy has prevented the genetic characterization of many E3 ubiquitin ligases in plants. Here, we performed a reverse genetic screen in Arabidopsis using a library of dominant-negative U-box-type E3 ubiquitin ligases to identify their roles in flowering time and reproductive development. We identified five U-box decoy transgenic populations that have defects in flowering time or the floral development program. We used additional genetic and biochemical studies to validate PLANT U-BOX 14 (PUB14), MOS4-ASSOCIATED COMPLEX 3A (MAC3A), and MAC3B as bona fide regulators of flowering time. This work demonstrates the widespread importance of E3 ubiquitin ligases in floral reproductive development. Furthermore, it reinforces the necessity of dominant-negative strategies for uncovering previously unidentified regulators of developmental transitions in an organism with widespread genetic redundancy, and provides a basis on which to model other similar studies. |
| Databáze: | OpenAIRE |
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