An electrochemical immunosensor for prostate specific antigen using nitrogen-doped graphene as a sensing platform
| Autor: | Wenjuan Zhang, Junjun Zhang, Wenwen Meng, Xi Chen, Yuzhong Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Chemistry Graphene General Chemical Engineering Aptamer 010401 analytical chemistry General Engineering 02 engineering and technology 021001 nanoscience & nanotechnology Electrochemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry law.invention chemistry.chemical_compound Ferrocene law Colloidal gold Differential pulse voltammetry 0210 nano-technology PSA Antibody Nuclear chemistry |
| Zdroj: | Analytical Methods. 11:2183-2189 |
| ISSN: | 1759-9679 1759-9660 |
| Popis: | In this study, a sensitive electrochemical immunosensor for prostate specific antigen (PSA) detection is described using nitrogen-doped graphene (NG) and gold nanoparticles (Au NPs) as a sensing interface. In this design, a PSA antibody (Ab) was first immobilized on the surface of NG decorated with Au NPs, and an aptamer labelled with ferrocene (Fc) was used as a signal probe. In the presence of PSA, a sandwich structure of Ab–PSA–aptamer was formed via specific recognition. Differential pulse voltammetry (DPV) was employed to record the electrochemical signal of Fc in phosphate-buffered saline (PBS) containing 0.10 M NaClO4. Under the optimal experimental conditions, the electrochemical immunosensor displayed a wide linear response range (0.05 ng mL−1 to 100 ng mL−1) and low detection limit (0.017 ng mL−1 (at S/N = 3)). Further, the immunosensor was evaluated with a serum sample, and satisfactory results were obtained. |
| Databáze: | OpenAIRE |
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