Meiotic Recombination in the Giraffe (G. reticulata)
| Autor: | Svatava Kubickova, Miluse Vozdova, Jiri Rubes, Jan Fröhlich, Hana Sebestova |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics biology Pseudoautosomal region Chromosome Reticulated giraffe Context (language use) biology.organism_classification 03 medical and health sciences Synaptonemal complex 030104 developmental biology Meiosis Nucleolus organizer region Homologous recombination Molecular Biology Genetics (clinical) |
| Zdroj: | Cytogenetic and Genome Research. 152:73-80 |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000478684 |
| Popis: | Recently, the reticulated giraffe (G. reticulata) was identified as a distinct species, which emphasized the need for intensive research in this interesting animal. To shed light on the meiotic process as a source of biodiversity, we analysed the frequency and distribution of meiotic recombination in 2 reticulated giraffe males. We used immunofluorescence detection of synaptonemal complex protein (SYCP3), meiotic double strand breaks (DSB, marked as RAD51 foci) in leptonema, and crossovers (COs, as MLH1 foci) in pachynema. The mean number of autosomal MLH1 foci per cell (27), which resulted from a single, distally located MLH1 focus observed on most chromosome arms, is one of the lowest among mammalian species analysed so far. The CO/DSB conversion ratio was 0.32. The pseudoautosomal region was localised in the Xq and Yp termini by FISH and showed an MLH1 focus in 83% of the pachytene cells. Chromatin structures corresponding to the nucleolus organiser regions were observed in the pachytene spermatocytes. The results are discussed in the context of known data on meiosis in Cetartiodactyla, depicting that the variation in CO frequency among species of this taxonomic group is mostly associated with their diploid chromosome number. |
| Databáze: | OpenAIRE |
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