| Autor: |
R. Griesemer, I.B. Rubin, P. Nettesheim, Walden E. Dalbey, Michael R. Guerin, John E. Caton, J. Kendrick, W. L. Maddox |
| Rok vydání: |
1976 |
| Předmět: |
|
| Zdroj: |
Toxicology and Applied Pharmacology. 37:557-569 |
| ISSN: |
0041-008X |
| DOI: |
10.1016/0041-008x(76)90217-9 |
| Popis: |
The feasibility of using rats in a bioassay for the effects of fresh tobacco smoke was investigated by a series of acute and long-term inhalation studies. Female SPF rats were exposed to 10% smoke from standard test cigarettes on an intermittent smoke machine. [14C]dotriacontane was used as label for the smoke particles, and it was calculated that under the described exposure conditions 0.3-0.4 mg of total particulate matter was retained in the rats from exposure to the smoke of one cigarette. This is similar to the dose sustained by hamsters under identical conditions. It was found that respiratory minute volume is decreased to 25% of normal during smoke exposure. The great sensitivity of rats to smoke toxicity could be overcome by gradual adaptation. This made it possible to chonically expose rats to 7 and 10 cigarettes per day with a death rate of 10 and 20%, respectively, at 12 months, and of 18 and 53%, respectively, at 18 months of exposure. This represents a far better survival rate at high smoke exposure levels for rats (or hamsters) than has ever been reported in the literature. Smoke-induced pathological changes were observed in the respiratory tract only. These included mild focal hyperplasia of the upper airways, bronchiolitis of the terminal bronchioles, and severe focal alveolitis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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