The effect of pyruvate on antibody interaction with group-specific erythrocyte antigens
| Autor: | N. S. Nefedova, O A Gusyakova, E. A. Shahnovich, F N Gylmiyarova, E. A. Ryskina, Radomskaya Viktoriya M, Yu. Y. Pervova, N. A. Kolotyeva, G. M. Baisheva, A. P. Pleten, I. F. Sidorova |
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| Rok vydání: | 2014 |
| Předmět: |
biology
medicine.diagnostic_test medicine.drug_class Metabolite Clinical Biochemistry Biological activity Monoclonal antibody Biochemistry Molecular biology Flow cytometry chemistry.chemical_compound Sodium pyruvate chemistry Antigen ABO blood group system medicine biology.protein Molecular Medicine Antibody |
| Zdroj: | Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry. 8:260-266 |
| ISSN: | 1990-7516 1990-7508 |
| Popis: | Using the AB0 antibody-antigen model the influence of natural metabolite pyruvate on the antibody interaction with of erythrocyte antigens, defining their group specificity has been investigated. Before agglutination reaction erythrocytes of A (II)–AB (IV) blood groups, monoclonal anti-A and anti-B antibodies were incubated with sodium pyruvate. Visualization of agglutinates was performed by means of flow cytometry and laser scanning confocal microscopy. Computer-aided prediction of the spectrum of biological activity of pyruvate by a PASS program proposed major regulatory pathways, in which pyruvate may be involved. It has been demonstrated that pyruvate can regulate the intensity of antigen-antibody interaction. These results suggest the possibility of using small molecules, for example pyruvate, as molecular probes and prospects of the use of erythrocytes with antigenic determinants of the ABO system expressed on their membranes for studies of protein-protein interactions due to convenient visualization and possibility of quantitative evaluation of this process. |
| Databáze: | OpenAIRE |
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