Collagen-based hydrogel functionalized with rhBMP-2

Autor:	E. O. Osidak, A. V. Vasilyev, D. V. Goldshtein, S. P. Domogatsky, T B Bukharova, E. V. Galitsyna
Rok vydání:	2020
Předmět:	Chemistry
Zdroj:	IOP Conference Series: Earth and Environmental Science. 548:062069
ISSN:	1755-1315 1755-1307
DOI:	10.1088/1755-1315/548/6/062069
Popis:	The study showed the cytocompatibility, matrix, and osteoinductive properties of collagen-fibronectin hydrogel impregnated with rhBMP-2. After 7 days of MSCs incubated with osteoplastic material the cell viability was 93.4 ± 3.6%. Expression of osteoblast-related genes after 14 days was increased by 1.4-4.8 times. Furthermore alkaline phosphatase activity and calcium ion quantity in cell lysates were increased by 2 and 2.7 times. It was accompanied by extracellular matrix mineralization. The results indicate that rhBMP-2 fully retains its activity inside the collagen-based material. The use of rhBMP-2 at a concentration of 1 μg/ml was ineffective and unable to induce osteogenic differentiation of MSCs. An effective osteoinductive concentration of rhBMP-2 was determined as 10 μg/ml.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d5d45aff6511647a57424f3f7744cd10 https://doi.org/10.1088/1755-1315/548/6/062069 Zobrazit plný text záznamu