| Autor: |
Wang Guangming, Sun Ruitao, Lidong Zeng, Meng Zhang, Jinsen Cai, Bo Yang, Nannan Zhao, Haitao Dan, Meirong Chen, Luo Weiwei, Hui Lv, Jianglin Chen, Zhou Zhiliang, Dexia Chen, Yanhua Zhang, Jiang Zefei, Jicai Fan, Qi Jifang, Siyu Liu, Zhang Juan, Wang Wen, Wu Ping, Li Gailing, Bin Liu, Yan Qin, Lu Yongyi, Chen Weiyue, Bo Zhou, Yan Li, Zheng Jiao, Yan Feng, Qi Wang, Wei Huang, Huan Shang, Fang Chen, Lei Liu, Huan Jin, Liuyan Lu, Liu Lichun, Jianfeng Xu, Xuesen He, Bing Xie, Li Linsen, Lin Xia, Sun Lei |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.1101/2020.09.28.20203455 |
| Popis: |
With the rapid development of precision medicine industry, DNA sequencing becomes increasingly important as a research and diagnosis tool. For clinical applications, medical professionals require a platform which is fast, easy to use, and presents clear information relevant to definitive diagnosis. We have developed a single molecule desktop sequencing platform, GenoCare™ 1600. Fast library preparation (without amplification) and simple instrument operation make it friendlier for clinical use. Here we presented sequencing data of E. coli sample from GenoCare™ 1600 with consensus accuracy reaches 99.99%. We also demonstrated sequencing of microbial mixtures and COVID-19 samples from throat swabs. Our data show accurate quantitation of microbial, sensitive identification of SARS-CoV-2 virus and detection of variants confirmed by Sanger sequencing. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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