| Popis: |
Publisher Summary M11X cells, formed by the fusion of mouse erythroleukemia cells (MEL) and human fibroblasts, contain the gene for human β-globin. Upon induction with HMBA, 10S or longer mRNA transcribed from the human β-globin gene is detected by Northern blot hybridization analysis. No human β-globin translation product is found using the acid–urea polyacrylamide gel electrophoresis, radioimmune assay, or amino acid sequence analysis of labeled globins. Although transcription occurs, an improper initiation or processing of the transcript has been suggested. The mechanism of this defect is being investigated by S1 mapping and sequence analysis of the mRNA. Studies are underway using a tridecanucleotide primer complementary to the middle of the mRNA to determine the primary sequence of the 5T end of the M11X mRNA. The primed reverse transcriptase sequencing technique is also being used to determine the specific primary sequence defects in the mRNA obtained from patients with β-thalassemia. |
