Isolating, Amplifying and Quantifying Sperm DNA inAnastrepha suspensa(Diptera: Tephritidae)
| Autor: | Ann H. Fritz, Gary N. Fritz, Laura A. Kirby, Preeti Dhakal |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Storage organ
endocrine system Anastrepha suspensa biology urogenital system Zoology biology.organism_classification Sperm Female sperm storage Spermatheca Insect Science Sexual selection Tephritidae Botany Sperm competition reproductive and urinary physiology Ecology Evolution Behavior and Systematics |
| Zdroj: | Florida Entomologist. 93:63-72 |
| ISSN: | 1938-5102 0015-4040 |
| Popis: | A major obstacle for many studies examining sperm competition and cryptic female choice in insects has been the identification and quantification of sperm stored in the sperm storage organs of females that have mated with two or more males. Historically, sexual selection studies have focused primarily on paternity outcomes for inferring potential underlying mechanisms (e.g., sperm competition and cryptic female choice). We describe a technique for isolating, genotyping and quantifying sperm in Anastrepha suspensa Loew, a species that has four sperm storage organs (three spermathecae and a ventral receptacle) that are minute (approximately 80 µm) and exhibit complex interior structures restricting sperm recovery through simple dissection. With our protocol, we were able to isolate and amplify sperm DNA (PCR of microsatellite loci) without contamination from female cells, and quantify sperm contributed to a storage organ by one or more males. Briefly, sperm storage organs are dissected-out of the f... |
| Databáze: | OpenAIRE |
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