13C/31P NMR Assessment of Mitochondrial Energy Coupling in Skeletal Muscle of Awake Fed and Fasted Rats
| Autor: | Kevin S. Cadman, Sylvie Dufour, Beat M. Jucker, Gerald I. Shulman, Xueying Cao, Jian-Ming Ren, Stephen F. Previs |
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| Rok vydání: | 2000 |
| Předmět: |
Messenger RNA
medicine.medical_specialty Skeletal muscle Cell Biology Hindlimb Mitochondrion Biology Biochemistry chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Internal medicine medicine Uncoupling protein Carnitine O-palmitoyltransferase Molecular Biology Adenosine triphosphate UCP3 |
| Zdroj: | Journal of Biological Chemistry. 275:39279-39286 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m007760200 |
| Popis: | To examine the relationship between mitochondrial energy coupling in skeletal muscle and change in uncoupling protein 3 (UCP3) expression during the transition from the fed to fasted state, we used a novel noninvasive31P/13C NMR spectroscopic approach to measure the degree of mitochondrial energy coupling in the hind limb muscles of awake rats before and after a 48-h fast. Compared with fed levels, UCP3 mRNA and protein levels in the gastrocnemius increased 1.7- (p |
| Databáze: | OpenAIRE |
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