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The study was conducted to investigate the effects of soybean meal (SBM), raffinose and stachyose on juvenile crucian carp (Carassius auratus gibelio♀ × Cyprinus carpio♂). The experimental diets consisted of one control diet based on fish meal (FM), one diet containing 300 g kg−1 SBM and four FM-based diets with the addition of either 6.7 g kg−1 raffinose (Raf), 33.9 g kg−1 stachyose (Sta), a combination of raffinose and stachyose (Raf−Sta) and finally a Raf−Sta diet supplemented with 2.5 g kg−1 saponins (Raf−Sta−Sap). After 3 weeks of feeding, the relative gut lengths of SBM-fed fish and the fish fed stachyose-containing diets were shorter than those of the FM-fed fish; further, more SBM-fed fish showed fissures on the tips of the intestinal folds. After 8 weeks of feeding, the growth of SBM-fed fish was significantly lower than that of FM-fed fish (P |
