Quantitative structure-retention relationships of antimicrobial hydrazides evaluated by reverse-phase liquid chromatography
| Autor: | Carlos A. Montanari, Maria Luiza C. Montanari, Quezia B. Cass |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Quantitative structure–activity relationship
Chromatography Molecular model Chemistry Organic Chemistry Clinical Biochemistry Intermolecular force Quantitative structure Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry Phase (matter) Volume fraction |
| Zdroj: | Chromatographia. 51:722-726 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1007/bf02505411 |
| Popis: | Quantitative structure-retention relationships of antimicrobial hydrazides were investigated by reversedphase liquid chromatography (RPLC), to disclose retention mechanisms operating in a C-8 column. Capacity factors (logk) were obtained using methanol-water mobile phase in volume fractions: 25–75%v/v. Extrapolated logk w values were derived from logk versus mobile phase, volume fraction of organic modifier, φ, using logk=logk w −Sφ. The chemical validity of a linear relationship between the slopes (S) and intercepts (logk w ) was established.S-logk w correlations are chemically meaningful for a non-homologous series of compounds. The existence of a clear separation of hydrazides into two different sets of hydrogen-bonding capabilities demonstrated the role of such relationships. Standard CoMFA fields were used to delineate those specific intermolecular interactions responsible for changes in retention of study hydrazides. Thus, a 3D QSAR (CoMFA) model showed thatS exhibits electrostatic behaviour towards a C-8 HPLC column. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink