PURIFICATION AND CHARACTERIZATION OF PHENOLOXIDASE ISOFORMS FROM TAIWANESE BLACK TIGER SHRIMP (PENAEUS MONODON)

Autor:	Maurice R. Marshall, R.S. Rolle, C.I. Wei, Nejib Guizani, J. S. Chen, J. S. Yang
Rok vydání:	1991
Předmět:	Pharmacology chemistry.chemical_classification Gene isoform biology Decapoda Ecology Hydrophilic interaction chromatography Biophysics Cell Biology biology.organism_classification Penaeus monodon Enzyme chemistry Biochemistry Prawn Moiety Ammonium sulfate precipitation Food Science
Zdroj:	Journal of Food Biochemistry. 15:17-32
ISSN:	1745-4514 0145-8884
DOI:	10.1111/j.1745-4514.1991.tb00141.x
Popis:	Two phenoloxidase (PPO) isoforms were purified from Taiwanese black tiger shrimp (Penaeus monodon) purified via ammonium sulfate precipitation and high performance hydrophobic interaction chromatography on phenyl Sepharose CL-4B. Optimal DL-a-3, 4-dihydroxyphenylalanine (D, L-DOPA) oxidation by these isoforms was observed to occur at pH 6.0, and at 45°C. The isoforms showed both mono- and di-PPO activities, and preferential medabolism of both monoand di-phenolic substrates lacking a carboxyl group either directly attached to the ring, or in the side chain moiety of the phenolic structure.
Databáze:	OpenAIRE
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