An Aptasensor Based on Polymer-Gold Nanoparticle Composite Microspheres for the Detection of Malathion Using Surface-Enhanced Raman Spectroscopy
| Autor: | Francisco Barahona, Adrienne Phifer, Cameron L. Bardliving, Carl A. Batt, John G. Bruno |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Materials science Ethylene glycol dimethacrylate Nanoparticle Nanotechnology Polymer Surface-enhanced Raman spectroscopy symbols.namesake chemistry.chemical_compound chemistry Dynamic light scattering Chemical engineering Colloidal gold Precipitation polymerization symbols Raman spectroscopy Biotechnology |
| Zdroj: | Industrial Biotechnology. 9:42-50 |
| ISSN: | 1931-8421 1550-9087 |
| DOI: | 10.1089/ind.2012.0029 |
| Popis: | We present a new format of apta-sensing composite particles for Surface-Enhanced Raman Spectroscopy (SERS) detection of malathion. These apta-sensing microspheres acquire their extraction capability through aptamer-target analyte interaction and Raman signal enhancement for SERS detection of the pesticide. Micron-sized polymer particles were synthesized by precipitation polymerization using methacrylic acid and ethylene glycol dimethacrylate as co-monomers in acetonitrile. Conjugation with colloidal gold nanoparticles (AuNPs) via modification with 2-aminoethanethiol led to polymer-AuNP composites with controlled aggregation of AuNPs onto the polymer surface. The thiolated aptamer targeting malathion was attached to the metal surface by thiol-gold interaction, resulting in polymer-AuNP-aptamer composite microspheres. The new material was characterized using two techniques: scanning electron microscopy imaging and dynamic light scattering measurements. The polymer-AuNP-aptamer particles were incuba... |
| Databáze: | OpenAIRE |
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