| Autor: |
A. Munnoz De La Pena, Anunciación Espinosa-Mansilla, F. Cañada Cañada, A. Jiménez Girón, Alejandro C. Olivieri, Isabel Durán-Merás |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Analytica Chimica Acta. 622:94-103 |
| ISSN: |
0003-2670 |
| DOI: |
10.1016/j.aca.2008.05.079 |
| Popis: |
The determination of folic acid and its two main serum metabolites, 5-methyltetrahydrofolic acid and tetrahydrofolic acid, has been accomplished using four-way data modelled by the third-order multivariate calibration methods unfolded and N-dimensional partial least-squares (U-PLS and N-PLS), in combination with the separate procedure known as residual trilinearization (RTL). The four-way data were acquired by following the photochemical reaction of these compounds by on line irradiation with a UV lamp. The excitation-emission matrices (EEMs) were recorded as a function of the irradiation time, using a fast scanning spectrofluorimeter. The method achieves selectivity from the different rates at which the corresponding photoproducts of the folic acid derivatives are formed and degraded. Several N-dimensional chemometric algorithms were used and the method was applied to the determination of these compounds in serum samples. The best algorithms to perform the multivariate calibration were U-PLS and N-PLS in combination with the separate residual trilinearization procedure, achieving the second-order advantage. The approach allows minimizing or eliminating traditionally time-consuming sample pre-treatments and can facilitate quantifying an analyte in its native environment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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