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Polyunsaturated fatty acid (PUFA) and eicosapentaenoic acid (EPA) are essential for the health of aquatic organisms and human beings. In order to optimize the culture conditions of Chaetoceros lorenzianus, effects of different iron sources (FeC6H5O7, FeCl3, FeSO4) and different iron concentrations (0.10 mg L− 1, 0.25 mg L− 1, 0.50 mg L− 1, 0.75 mg L− 1, 1.00 mg L− 1, 1.25mg L− 1) on C. lorenzianus were studied. The results showed that different iron sources and different iron concentrations had significant effects on the biomass, chlorophyll A, total lipid and fatty acids of C. lorenzianus (P 4 and FeC6H5O7, FeCl3 had better effects on the growth, chlorophyll a, total lipids, and n-3 PUFA (EPA and DHA) content of C. lorenzianus. The optimum concentration of FeCl3 for the growth and synthesis of chlorophyll a in C. lorenzianus was 0.75–1 mg L− 1, and the optimum concentration for lipid accumulation was 0.25 mg L− 1. The secondary culture method can be used in large-scale culture, that is, the initial culture is performed under the optimal iron concentration (0.75mg L− 1 FeCl3) for obtaining higher biomass, and then, the subsequent culture is performed under a low iron concentration(0.25 mg L− 1 FeCl3) to accumulate higher total lipids. |
