Genetic Analysis of Fluorescent Labeled Amplified DNA Using Series of Oligonucleotide Probes Covalently Bound to Membranes

Autor:	M. G. Ivanovskaya, A. N. Belozersky, I. V. Lebedeva, Nikolai Naryshkin, Shabarova Za, K. I. Ivanov, I. A. Kozlov
Rok vydání:	1995
Předmět:	chemistry.chemical_classification chemistry.chemical_compound chemistry Oligonucleotide Point mutation Nucleotide A-DNA Oligomer restriction Genetic analysis Molecular biology Fluorescence DNA
Zdroj:	Acta Medicinæ Legalis Vol. XLIV 1994 ISBN: 9783540588474
DOI:	10.1007/978-3-642-79523-7_16
Popis:	Recently proposed reverse hybridization method is very convenient for simultaneous detection of a set nucleotide sequences in a DNA fragment of interest (1,2). The main idea of this method is immobilization of an oligonucleotide probes set on a solid support and their hybridization with the labeled DNA fragment. Here we report on an improved non-radioactive reverse hybridization method and provide an example of its application for testing point mutations responsible for β-thalassemia.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d52f72d7678dffdd2db25d9fa5e0e516 https://doi.org/10.1007/978-3-642-79523-7_16 Zobrazit plný text záznamu