Indapamide: Measurement by a Fluorescence Assay

Autor:	Peter E. Grebow, Martha M. Johnston, L B Mellett
Rok vydání:	1977
Předmět:	Aqueous solution Chromatography business.industry Calibration curve Fluorescence assay Indapamide Extraction (chemistry) General Medicine Pharmacology Fluorescence Yield (chemistry) medicine business medicine.drug
Zdroj:	Current Medical Research and Opinion. 5:9-12
ISSN:	1473-4877 0300-7995
DOI:	10.1185/03007997709110218
Popis:	SummaryA fluorescence assay for indapamide which is both sensitive and capable of handling large numbers of samples has been developed. Reaction of indapamide with NaOH at 100°C has been shown to yield a fluorescent product, which has excitation and emission wavelengths of 284 nm and 356 nm, respectively. The assay is sensitive to levels of indapamide of 0.025 μg/ml in aqueous solutions, and a linear calibration curve between 0.025 μg/ml and 2 μg/ml is observed. The adaptability of this assay to urinary samples of the drug has been demonstrated by the development of a selective extraction procedure. Using this procedure, there was no interference in the assay from endogenous urinary compounds.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d51aa3b40ca208bdde884d24db095f44 https://doi.org/10.1185/03007997709110218 Zobrazit plný text záznamu