| Autor: |
Hans-Georg Sydow, Nikoloz Tsikolia, Christoph Viebahn, Tobias Pieper |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Avian and Reptilian Developmental Biology ISBN: 9781493972159 |
| DOI: |
10.1007/978-1-4939-7216-6_21 |
| Popis: |
Appropriate mechanical tension of the vitelline membrane as the culture substrate for the early chick embryo is frequently reported to be required for successful in vitro development. Here we describe a modified device, made of anodized aluminum, for in vitro culture which we used for studies of left-right symmetry breaking with emphasis on morphology and gene expression as readouts. The technique allows for easy, high-throughput tissue handling and provides a suitable tension in a stable and easily reproducible manner proven to be suitable for correct molecular left-right patterning and heart looping after long-term culture. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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