Methemoglobin and the Activities of Catalase and Superoxide Dismutase in Nucleated Erythrocytes of Scorpaena porcus (Linnaeus, 1758) under Experimental Hypoxia (in vitro)
| Autor: | A. A. Soldatov, T. I. Andreyenko, T. A. Kukhareva, A. Y. Andreeva |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Reactive oxygen species 030102 biochemistry & molecular biology biology Chemistry Biophysics Nucleated Red Blood Cell Molecular biology Methemoglobin Superoxide dismutase 03 medical and health sciences Red blood cell 030104 developmental biology medicine.anatomical_structure Catalase hemic and lymphatic diseases medicine biology.protein Limiting oxygen concentration Hemoglobin |
| Zdroj: | Biophysics. 65:452-459 |
| ISSN: | 1555-6654 0006-3509 |
| DOI: | 10.1134/s0006350920030197 |
| Popis: | The effect of hypoxia on nucleated red blood cells of the black scorpionfish (Scorpaena porcus) was studied in vitro. Deep hypoxia (the oxygen concentration was less than 1 mg O2 L–1; the norm was 7–8 mg O2 L–1) led to the transition of a part of the hemoglobin molecules to the ferric state (methemoglobin). The maximum increase in the concentration of methemoglobin was 32%. The accumulation of methemoglobin in red blood cells was accompanied by an increase in the activity of catalase and superoxide dismutase and a decrease in the content of reactive oxygen species in the cytoplasm of cells. It was shown that the formation of methemoglobin did not cause damage to the cytoplasmic membranes of red blood cells. The percentage of red blood cell lysis in deoxygenated (less than 1.0 mg O2 L–1) suspensions quantitatively coincided with the control values. |
| Databáze: | OpenAIRE |
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