Analysis of Isoflavones from Korean and Chinese Soybean and Processed Products by HPLC

Autor: Kyung-Ho Row, Jinzhu Zheng, Yinzhe Jin
Rok vydání: 2005
Předmět:
Zdroj: Journal of the Korean Chemical Society. 49:349-354
ISSN: 1017-2548
DOI: 10.5012/jkcs.2005.49.4.349
Popis: The four Isoflavones of daidzin, genistin, daidzein and genistein were extracted from Korean and Chinese soybean, fermented soybeans and soybean paste. The extracted samples were analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). The mobile phase was applied to the binary system of A (water/acetic acid, 99.9/0.1, vol%) and B (acetonitrile/acetic acid, 99.9/0.1, vol%) from 85:15 (A: B vol%) to 65:35 (A: B vol%) in a gradient time of 50 min. The amount of isoflavones extracted from the Chinese soybean was extracted 42.6% more than that from the Korean soybean. For the Korean fermented soybean, the contents of the four isoflavones were more than 1.6 times of that from the soybeans. However, a slight amount of isoflavones was found in the soybean paste.
Databáze: OpenAIRE