A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli
| Autor: | Renaud Vincentelli, Joana L. A. Brás, Ana Filipa Sequeira, Catarina I. P. D. Guerreiro, Carlos M. G. A. Fontes, Vânia O. Fernandes |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cloning Expression vector Recombinant expression Oligonucleotide Computer science Mutagenesis Heterologous Mutagenesis (molecular biology technique) Computational biology medicine.disease_cause DNA sequencing law.invention 03 medical and health sciences 030104 developmental biology law Recombinant protein production medicine Nucleic acid Recombinant DNA Protein biosynthesis Codon optimization Escherichia coli Function (biology) |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493970599 |
| DOI: | 10.1007/978-1-4939-7060-5_7 |
| Popis: | Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenesis procedures and allows generating nucleic acids for which no template is available. Here, we describe a high-throughput platform to design and produce multiple synthetic genes ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|