Synthetic conformational antigen which simulates the extracellular part of the M2-muscarinic receptor: interaction with blood sera of patients suffering from idiopathic arrhythmias

Autor: N. A. Mironova, M M Rogova, Kirill Zykov, Zh. D. Bespalova, A. S. Molokoedov, Maria Sidorova, E E Efremov, Sergey P. Golitsyn, Pal'keeva Me, T V Sharf, E. N. Mamochkina, A. A. Az’muko
Rok vydání: 2013
Předmět:
Zdroj: Russian Journal of Bioorganic Chemistry. 39:252-258
ISSN: 1608-330X
1068-1620
Popis: Fragments corresponding to the 83–98 sequence of the first extracellular loop and to the 168–192 and 171–182 sequences of the second extracellular loop of the M2-muscarinic receptor (antibodies to this receptor could be markers of early symptoms of heart disorders) were synthesized by solid phase method using the Fmoc-SPPS strategy. A new conformational antigen with the natural location of the disulfide bridge was prepared by selective formation of disulfide bond between the corresponding cysteine residues in the synthe-sized peptides and characterized. The comparative analysis of reactivity of the synthesized peptides towards sera from patients which had no organic heart disease was performed. A new conformational antigen was effectively bound to the sera from patients with idiopathic arrhythmias, but without symptoms of organic heart disease.
Databáze: OpenAIRE
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