Mycobacterium tuberculosisComplex Lineage 3 as Causative Agent of Pulmonary Tuberculosis, Eastern Sudan1
| Autor: | Elvira Richter, Mohammed A. Bakheit, Eltahir A G Khalil, Ulrich E. Schaible, Doris Hillemann, Katharina Kranzer, Lothar H. Wieler, Stefan Niemann, Matthias Merker, Yassir Adam Shuaib, Glennah Kerubo, M. A. Abdalla, Sönke Andres, Saad El-Tiab Mohamed-Noor |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Whole genome sequencing Tuberculosis Epidemiology 030231 tropical medicine Drug resistance Biology medicine.disease biology.organism_classification Virology Multiple drug resistance 03 medical and health sciences 0302 clinical medicine Infectious Diseases Antibiotic resistance Mycobacterium tuberculosis complex medicine Sputum 030212 general & internal medicine medicine.symptom Pathogen |
| Zdroj: | Emerging Infectious Diseases. 26:427-436 |
| ISSN: | 1080-6059 1080-6040 |
| Popis: | Pathogen-based factors associated with tuberculosis (TB) in eastern Sudan are not well defined. We investigated genetic diversity, drug resistance, and possible transmission clusters of Mycobacterium tuberculosis complex (MTBC) strains by using a genomic epidemiology approach. We collected 383 sputum specimens at 3 hospitals in 2014 and 2016 from patients with symptoms suggestive of TB; of these, 171 grew MTBC strains. Whole-genome sequencing could be performed on 166 MTBC strains; phylogenetic classification revealed that most (73.4%; n = 122) belonged to lineage 3 (L3). Genome-based cluster analysis showed that 76 strains (45.9%) were grouped into 29 molecular clusters, comprising 2-8 strains/patients. Of the strains investigated, 9.0% (15/166) were multidrug resistant (MDR); 10 MDR MTBC strains were linked to 1 large MDR transmission network. Our findings indicate that L3 strains are the main causative agent of TB in eastern Sudan; MDR TB is caused mainly by transmission of MDR L3 strains. |
| Databáze: | OpenAIRE |
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