| Autor: |
J. Y. Yong, M. Sinniah, M. K. Brian Cheong, N. A. Mokhtar, T. Joash |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Journal of Health and Translational Medicine. :1-5 |
| ISSN: |
2289-392X |
| DOI: |
10.22452/jummec.sp2020no1.2 |
| Popis: |
Reverse transcriptase polymerase chain reaction (RT-PCR) assays for coronavirus disease 2019 (COVID-19) should be interpreted with clinical, epidemiological history and exposure risk to avoid misdiagnosis. We report a cruise-ship worker with significant travelling history, presented with acute respiratory symptoms and radiographic evidence of viral pneumonia. Initial RNA-dependent RNA polymerase (RdRp) gene confirmatory assay was negative. Use of a more robust RT-PCR assay detected ORF1ab, N and S genes for COVID-19, and the diagnosis was supported by an IgM and IgG positive COVID-19 serology. Subsequent follow up samples which reported inconsistencies in detecting RdRp gene also raise the concern of reliability of RdRp gene as the confirmatory assay for diagnosis of COVID-19. Patient later had prolonged viral shedding beyond serological recovery, with a negative viral culture reflecting non-infectivity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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