Toxicity, Clastogenicity and Genotoxicity of Theophylline and Pentoxifylline in Mammalian Cells Cultured In Vitro
| Autor: | Eva Horváthová, Ivan Chalupa, Alena Gábelová, M. Blasko, E. Bozsakova, Darina Slamenova |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Genetics
General Medicine respiratory system 030204 cardiovascular system & hematology Biology Pharmacology Toxicology medicine.disease_cause General Biochemistry Genetics and Molecular Biology In vitro Pentoxifylline 03 medical and health sciences Medical Laboratory Technology Clastogen 0302 clinical medicine Mechanism of action Toxicity medicine Theophylline 030212 general & internal medicine medicine.symptom Cytotoxicity Genotoxicity medicine.drug |
| Zdroj: | Alternatives to Laboratory Animals. 23:504-512 |
| ISSN: | 2632-3559 0261-1929 |
| DOI: | 10.1177/026119299502300414 |
| Popis: | — As part of a developmental study on theophylline and pentoxifylline, these drugs were tested for possible cytotoxic, mutagenic and clastogenic effects on V79 hamster cells and human lymphocytes cultured in vitro. After the short-term treatment of V79 cells with theophylline and pentoxifylline, the cells were relatively resistant to the toxic effects of these methylxanthines. Generally, only high concentrations of theophylline or pentoxifylline had toxic effects on exposed V79 cells. Short-term treatment of V79 cells with theophylline or pentoxifylline did not induce 6-thioguanine resistant mutations in either the presence or absence of S9 fractions. However, in the absence of an S9 fraction, elevated levels of single-strand breaks in DNA were induced. Both methylxanthines caused clastogenic effects in human lymphocytes and hamster V79 cells after long-term exposure. We suggest that theophylline and pentoxifylline are clastogenic but not genotoxic, and that the molecular mechanism for the induction of single-strand breaks in DNA and the induction of chromosomal aberrations in cells treated with theophylline and pentoxifylline is not the induction of lesions in the DNA but the inhibition of DNA chain elongation. |
| Databáze: | OpenAIRE |
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