Virulence Factor Profiles of Escherichia coli O157:H7 Bacteriophage Isolates from Sewage and Livestock Stools
| Autor: | Jina Seo, Min Hwa Lee, Changsun Choi, Dong Joo Seo, Mi Hwa Oh, Su Been Jeon, Hyejin Oh |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
biology
business.industry animal diseases Sewage Virulence biology.organism_classification medicine.disease_cause Virology Virulence factor nervous system diseases Microbiology law.invention Bacteriophage fluids and secretions immune system diseases law STX2 medicine Livestock business Escherichia coli Polymerase chain reaction |
| Zdroj: | Journal of Food Hygiene and Safety. 29:316-321 |
| ISSN: | 1229-1153 |
| DOI: | 10.13103/jfhs.2014.29.4.316 |
| Popis: | The aim of study was to investigate the virulence profile of Escherichia coli O157:H7 bacterioph- ages isolated from sewage and livestock stools. Among 23 E. coli O157:H7 bacteriophages, 14 strains were isolated from sewage and 9 were from animal stools collected from 10 livestock farms in Korea. For each bacteriophage DNA sample, the presence of stx1, stx2, eae, aafII, ial, elt, estI, estII, astA, afa, and cnf was examined by polymerase chain reaction. The detection rate of eae, stx2, estI, astA, and ial was 100%, 69.6%, 13.0%, 13.0%, 8.7%, respectively. While all E. coli O157:H7 bacteriophages isolated from stools carried eae+stx2, stx2+eae, eae+astA, eae, stx2+eae+estI, eae+estI, stx2+eae+ial, and eae+ial were observed in bacteriophages isolated from sewage. As sev- eral plasmid-carrying virulence factors (estI, astA, and ial) were found in E. coli O157:H7 bacteriophages obtained from sewage and stools, the microbial safety of bacteriophages should be investigated in further study. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|