Comparison of Partial 16S rRNA Sequences of Different Helicobacter pylori Strains, Helicobacter mustelae and a Gastric Campylobacter-like Organism (GCLO)
| Autor: | Timo U. Kosunen, Matti Kaartinen, Marja-Liisa Solin, Johanna Höök-Nikanne |
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| Rok vydání: | 1991 |
| Předmět: |
Genetics
0303 health sciences 030306 microbiology Nucleic acid sequence Sequence alignment Ribosomal RNA Biology Helicobacter pylori bacterial infections and mycoses biology.organism_classification 16S ribosomal RNA Applied Microbiology and Biotechnology Microbiology Campylobacter jejuni 03 medical and health sciences Phylogenetics Consensus sequence Ecology Evolution Behavior and Systematics 030304 developmental biology |
| Zdroj: | Systematic and Applied Microbiology. 14:270-274 |
| ISSN: | 0723-2020 |
| Popis: | Summary Partial 16S rRNA sequences of seven Helicobacter pylori strains from three different continents were compared. High homogeneity (99.5%–100%) of 16S rRNA between H. pylori strains was demonstrated, when a 397 nucleotides long stretch of RNA was compared. The obtained consensus sequence was practically identical with one of the two published sequences, 99.4% similarity ( Romaniuk et al., J. Bact. 169 , 2137–2141 (1987)), whereas with the other published sequence the identity was lower, 95.8% similarity ( Thompson et al., Int. J. System. Bact. 38 , 190–200 (1988)). Comparison of the consensus H. pylori sequence with the sequence of Helicobacter mustelae , the spiral organism isolated from ferrets, revealed 93.8% similarity. H. mustelae was also compared with published sequences of Wollinella succinogenes , and Campylobacter jejuni . The percentual sequence similarities were 96.0 and 83.7, respectively. A gastric campylobacter-like organism (GCLO, NCTC 11847), isolated from the gastric antrum has been demonstrated to resemble C. jejuni biochemically. By 16S rRNA sequences we demonstrate that the GCLO is in fact a C. jejuni strain. |
| Databáze: | OpenAIRE |
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