Role of vascular endothelial growth factor in angiodysplasia: An interventional study with thalidomide

Autor: Hong-hong Tan, Zhi-Zheng Ge, Shudong Xiao, Huimin Chen, Jing-Yuan Fang, Yun-Jie Gao, Chun-Hong Xu, Wenzhong Liu
Rok vydání: 2012
Předmět:
Zdroj: Journal of Gastroenterology and Hepatology. 27:1094-1101
ISSN: 0815-9319
DOI: 10.1111/j.1440-1746.2011.06967.x
Popis: Background and Aim: The pathogenesis of angiodysplasia is still not fully understood and effective therapy is not available. Thalidomide was reported to be effective in the treatment of angiodysplasia, but the mechanisms underlying its activity are, as yet, unknown. We aimed to investigate the expression of vascular endothelial growth factor (VEGF) in angiodysplasia tissues, and the role of hypoxia-inducible factor-1α (HIF-1α) and basic fibroblast growth factor (bFGF) on VEGF expression in human umbilical vein endothelial cells (HUVEC). Additionally, we aimed to study the role of thalidomide in these parameters. Methods: Immunohistochemistry was performed to visualize VEGF in angiodysplasia lesions. HUVEC were incubated under hypoxic conditions or in the presence of bFGF. Effects of exposure to thalidomide were studied. Cell growth was assessed in methylthiazolyte-trazolium assays. Enzyme-linked immunosorbent assays and real-time polymerase chain reaction were performed to assess the expression of VEGF at protein and mRNA levels. Western blot was performed to detect the expression of HIF-1α under hypoxic conditions. Results: VEGF was strongly expressed in 75% of patients with angiodysplasia lesions, as compared to expression in patients without angiodysplasia lesions. VEGF was also induced in HUVEC under hypoxic conditions (P
Databáze: OpenAIRE
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