Cryopreservation of apple (Malus domestica ‘Benoni’) dormant buds using two-step freezing method
| Autor: | J. I. Mir, Sandhya Gupta |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Acta Horticulturae. :323-328 |
| ISSN: | 2406-6168 0567-7572 |
| DOI: | 10.17660/actahortic.2019.1234.42 |
| Popis: | Apple is the most important temperate fruit in India. The top apple producing states are Jammu and Kashmir (JK ICAR-Central Institute of Temperate Horticulture, Srinagar (JK ICAR-Indian Agricultural Research Institute’s Regional Station, Shimla (HP); Dr. Y.S. Parmar University of Horticulture and Forestry, Solan (HP); Govind Ballabh Pant University of Agriculture and Technology, Pantnagar (UK); Sher-e-Kashmir University of Agricultural Science and Technology, Srinagar (J&K). To have a backup, some accessions are being maintained under in vitro genebank at New Delhi. For long-term conservation of apple accessions, cryopreservation is the only method. Dormant buds of apple (Malus domestica ‘Benoni’) were cryopreserved by two-step freezing method. Apple twigs were collected from Srinagar at the sub-freezing temperature. Twigs were stored at 5-6°C until use. The buds were scooped from the twigs and desiccated on silica gel. Then the buds were subjected to gradual low temperature treatments up to -30°C before submerging into liquid nitrogen. Programmable freezer was not used for freezing treatments. Post-thaw recovery (in vitro) was about 20%. The average rate of shoot formation was about 80%. This protocol of two-step freezing method was successfully applied to other three apple cultivars with the post-thaw in vitro recovery about 11-40%. This method appears to be a promising technique for cryopreserving dormant buds from field grown apple trees. |
| Databáze: | OpenAIRE |
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