On the mechanisms of deactivation of Bacillus atrophaeus spores using supercritical carbon dioxide
| Autor: | Karen F. Fox, Jian Zhang, Martine LaBerge, Nishita Dalal, Michael J. Drews, Alvin Fox, Courtney Gleason, Y. H. An, Michael A. Matthews, Lashanda N. Waller |
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| Rok vydání: | 2006 |
| Předmět: |
Supercritical carbon dioxide
biology General Chemical Engineering fungi Sterilization (microbiology) Condensed Matter Physics biology.organism_classification Dipicolinic acid Spore chemistry.chemical_compound chemistry Bacillus atrophaeus Biochemistry Transmission electron microscopy Physical and Theoretical Chemistry Hydrogen peroxide Nuclear chemistry |
| Zdroj: | The Journal of Supercritical Fluids. 38:268-273 |
| ISSN: | 0896-8446 |
| DOI: | 10.1016/j.supflu.2006.02.015 |
| Popis: | Greater than 6-log reduction of viable Bacillus atrophaeus spores is achieved by treatment with supercritical carbon dioxide and hydrogen peroxide. Spores treated with CO 2 + H 2 O 2 released significantly more dipicolinic acid than untreated or pure CO 2 treated spores. Substantial damage to the spore envelope was observed with transmission electron microscopy (TEM). These observations are consistent with damage to the outer layers of the spore and subsequent uptake of H 2 O 2 , resulting in death. |
| Databáze: | OpenAIRE |
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