Axon orientation by gradient of cytochalasin D inside microfluidic device
| Autor: | Seog Woo Rhee, Sanjeev Kumar Mahto, Liang Xiao |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Materials science
Microfluidics Biomedical Engineering Bioengineering Nanotechnology Orientation (graph theory) Soft lithography law.invention chemistry.chemical_compound medicine.anatomical_structure nervous system chemistry law medicine Biophysics Neuron Electrical and Electronic Engineering Axon Photolithography Concentration gradient Biotechnology Cytochalasin D |
| Zdroj: | BioChip Journal. 6:335-341 |
| ISSN: | 2092-7843 1976-0280 |
| DOI: | 10.1007/s13206-012-6405-z |
| Popis: | This paper describes the development of a microfluidic device for axon orientation. The device was fabricated with poly (dimethylsiloxane) by soft lithography and replica molding. There were two separated rooms in the device, and a control system and stable concentration gradient system were maintained in each room for at least 24 h. Finally, to demonstrate a practical application of the device, neuronal cells were cultured in both cell rooms, and the growth of cells and formation of axons were monitored under a concentration gradient of cytochalasin D for 8 h. Quantitative analysis of the orientation of axons was analyzed, and the data were plotted in a polar graph as a function of the direction. A figure of 33.8% of positive responding neurons were found in the cytochalasin D gradient room, while that figure was 23.3% of cells in control room. The extent of axon orientation shows a considerable difference between the two rooms, and the device developed here is capable of investigating axon orientation and can be used for other biological applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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