Transcription factor STAT1 promotes the proliferation, migration and invasion of nasopharyngeal carcinoma cells by upregulating LINC01160
| Autor: | Yexun Song, Xiang Qing, Jingang Ai, Guolin Tan, Shan-shan Xiong, Ru Gao, Tiansheng Wang, Wei Li |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cancer Research biology business.industry Cell growth Promoter Cell migration General Medicine medicine.disease 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Real-time polymerase chain reaction Oncology Nasopharyngeal carcinoma 030220 oncology & carcinogenesis Cancer research biology.protein Medicine STAT1 business Transcription factor Chromatin immunoprecipitation |
| Zdroj: | Future Oncology. 17:57-69 |
| ISSN: | 1744-8301 1479-6694 |
| DOI: | 10.2217/fon-2020-0618 |
| Popis: | Aim: To investigate the role of LINC01160 in nasopharyngeal carcinoma (NPC). Materials & methods: Using NPC cells CNE-2 and HNE-2 in vitro, we performed quantitative PCR to determine mRNA expression and western blotting to determine protein expression. CCK-8, transwell, flow cytometry and wound healing assays were done to examine the function of LINC01160 and STAT1. Chromatin immunoprecipitation PCR (ChIP-PCR) confirmed that STAT1 combines with the LINC01160 promoter region. Xenograft experiments were used to verify the role of STAT1 and LINC01160 in vivo. Results: LINC01160 is upregulated in NPC and can promote a malignant cell phenotype. STAT1 is a transcription factor of LINC01160 and can promote a malignant cell phenotype through upregulating LINC01160 expression. Conclusion: STAT1 can promote a malignant cell phenotype by upregulating LINC01160. |
| Databáze: | OpenAIRE |
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