STUDIES ON A NEW ANTITUMOR ANTIBIOTIC, LARGOMYCIN. II

Autor:	Toshikazu Miyagishima, Katsuyuki Nawa, Takehiko Yajma, Katsuo Kumagai, Totaro Yamaguchi, Nakao Ishida, Yukio Ito, Tatsuo Kashida, Tomoharu Okuda
Rok vydání:	1970
Předmět:	Pharmacology Ammonium sulfate Aqueous solution Chromatography Cellophane law.invention chemistry.chemical_compound Electrophoresis Isoelectric point Column chromatography chemistry law Sephadex Drug Discovery Polyacrylamide gel electrophoresis
Zdroj:	The Journal of Antibiotics. 23:373-381
ISSN:	1881-1469 0021-8820
DOI:	10.7164/antibiotics.23.373
Popis:	Isolation and purification of largomycin from the culture nitrate of Streptomyces pluricolorescens MCRL-0367 is described. The crude complex of largomycin was obtained from the culture nitrate by means of precipitation with ammonium sulfate. Three active components were separated by the following procedures : gradient extraction with aqueous ammonium sulfate solution, isoelectric point precipitation, preparative electrophoresis on polyacrylamide gel, AE-collulose column chromatography, gel-filtration on Sephadex G-100 and dialysis with a cellophane membrane. As a result, the most biologically active component, largomycin F-II, was obtained, the homogeneity of which was proved by ultracentrifugation and electrophoretic analyses. Largomycin F-II proved to be a chromoprotein of high molecular weight (25, 000) containing a pH-indicator chromophore in the molecule. The most unique characteristic of largomycin F-II, which differentiate it from similar antibiotics is the lack of hexosamine and cysteine in its molecule.
Databáze:	OpenAIRE
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