| Autor: |
Larissa Schemes Heinzelmann, Marcelo Pereira de Barros, Fernando Rosado Spilki, Rodrigo Staggemeier, Juliane Deise Fleck, GS Monteiro, Manoela Tressoldi Rodrigues, NK Rech, Mayra Cristina Soliman, Mariana Kluge |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Brazilian Journal of Biology. 75:11-16 |
| ISSN: |
1519-6984 |
| Popis: |
The spread of enteric viruses of domestic animals and human beings to wild species can be facilitated by the resistance of these viruses on the environment and their ability to be transmitted by water and contaminated food. The health status of the populations of pampas foxes (Lycalopex gymnocercus) and crab-eating foxes (Cerdocyon thous) is largely unknown and the landscapes occupied by these animals in southern Brazil have been threatened by human occupation and expansion of agriculture. In this work, the search of genomes of human and canine adenoviruses in feces from these wild carnivores was used to track the dissemination of domestic animals and human pathogens to the free-living populations in a wildlife reserve located in southern Brazil. This was performed by virus-specific differential real-time polymerase chain reactions (qPCR) on stool specimens, avoiding capture and additional stress to the animals. Genus-specific conventional reverse-transcriptase PCR (RT-PCR) was complementarily performed aiming the detection of enteroviruses (EV) and rotaviruses (RV) on these same samples. HAdV genomes were found on 14 out of the 17 (82.35%) stool samples analysed, whereas CAV was found co-infecting 5 of these samples. RV genomes were detected on 7 of the 17 samples (41.18%) and all samples were negative for EV. The results point to the dispersion of HAdV and RV at a high rate to these species of South American wild carnivores, which can be an effect of growing anthropisation of the habitat of these animals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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