Observation with microcalorimetry: Behaviour of P. aeruginosa in mixed cultures with S. aureus and E. coli
| Autor: | Anthony E. Beezer, Simon Gaisford, Mansa Fredua-Agyeman |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Isothermal microcalorimetry Chemistry Pseudomonas aeruginosa 030106 microbiology Heat output Condensed Matter Physics medicine.disease_cause 03 medical and health sciences Mixed culture Staphylococcus aureus medicine Pure culture Food science Physical and Theoretical Chemistry Instrumentation Nutrient broth Escherichia coli |
| Zdroj: | Thermochimica Acta. 663:93-98 |
| ISSN: | 0040-6031 |
| Popis: | In this work, isothermal microcalorimetry (IMC) was used to study the behaviour of mixed cultures of Pseudomonas aeruginosa with Staphylococcus aureus and Escherichia coli. The species were firstly studied as pure culture at 106 CFU/mL densities in nutrient broth (NB). The species were then introduced into NB to give an equivalent inoculum of 106 CFU/mL of each. Additionally, the inoculum density of one species was maintained at 106 CFU/mL and the other varied between 103–105 CFU/mL. The IMC power-time curves were characteristic for the species in the medium. The total heat output (Qt), amplitude of first peak (P1), time of registration of first peak (t1), final metabolic/maximum peak (Pfmax) and time of registration of the final metabolic/maximum peak (tfmax) were calculated from the power-time data. A mixed culture of P. aeruginosa and S. aureus at equal densities exhibited metabolism synonymous to P. aeruginosa alone. When the density of P. aeruginosa was decreased, S. aureus gradually recovered showing power-time profiles that demonstrated this. Mixed cultures of P. aeruginosa and E. coli at equal densities showed power-time profiles representative of both species. |
| Databáze: | OpenAIRE |
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