Isolation and characterization of polymorphic microsatellite loci for the gopher tortoise (Gopherus polyphemus) and cross-amplification in other species of Gopherus

Autor:	Brian R. Kreiser, Carl P. Qualls, Daniel L. Gaillard, Joshua R. Ennen
Rok vydání:	2012
Předmět:	Conservation genetics Loss of heterozygosity biology Tortoise Polyphemus Genetics Microsatellite Zoology biology.organism_classification Cross amplification Gopherus flavomarginatus Ecology Evolution Behavior and Systematics Gopherus berlandieri
Zdroj:	Conservation Genetics Resources. 5:255-259
ISSN:	1877-7260 1877-7252
Popis:	All members of the tortoise genus Gopherus have some degree of conservation concern. We report on the development of thirty-two new microsatellite loci for gopher tortoise, which were tested on 31 individuals from Ft. Benning, Georgia, USA. These loci possessed an average number of 5.09 alleles, an average observed heterozygosity of 0.574 and an average expected heterozygosity of 0.589. We also tested these loci in three congeners: Gopherus morafkai, Gopherus flavomarginatus and Gopherus berlandieri. The new microsatellite loci should prove useful in conservation efforts of Gopherus polyphemus and other Gopherus species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d45e6de6641e84374ad5fb48f10aca7c https://doi.org/10.1007/s12686-012-9781-8 Zobrazit plný text záznamu Full text from SpringerLink