Popis: |
The storage triacylglycerols of oil-rich seeds are hydrolyzed during germination of such seeds by the action of the endogenous lipase (triacylglycerol acylhydrolases, EC 3.1.1.3) and the lipolysis products, i. e. fatty acids and glycerol, are metabolized further in glyoxysomes and other organelles. It is well known that germinating seedlings of many oilseeds are rich source of lipases which have been isolated, purified and characterized from such tissues and some of these purified enzyme preparations have been used as catalyst in biotransformation of lipids [1,2]. Especially, the lipase from germinating seedlings of the very common oilseed — rape (Brassica napus L.) — has been isolated [3,4] partially purified [5,6] and used as biocatalyst after immobilization [7–10]. |