The green fluorescent protein as a visible marker for lactic acid bacteria in complex ecosystems

Autor:	L. Anne Glover, Harry J. Flint, Derry K. Mercer, Karen P. Scott
Rok vydání:	1998
Předmět:	Ecology biology Lactococcus lactis Streptococcus gordonii biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Marker gene Molecular biology Enterococcus faecalis Green fluorescent protein Aequorea victoria medicine Escherichia coli Bacteria
Zdroj:	FEMS Microbiology Ecology. 26:219-230
ISSN:	1574-6941 0168-6496
DOI:	10.1111/j.1574-6941.1998.tb00507.x
Popis:	The potential of the gfp (green fluorescent protein) gene from the jellyfish Aequoria victoria as a visible marker for tracking lactic acid bacteria introduced into complex anaerobic ecosystems was examined. A red-shifted mutant gfp gene was placed downstream of the constitutive Lactococcus lactis P32 promoter and fluorescence resulting from green fluorescent protein (GFP) expression was detected in Escherichia coli and L. lactis. The construct (pKPSPsgfp) was also introduced into Enterococcus faecalis, Streptococcus gordonii and Streptococcus bovis by mobilisation with pVA797. In all cases fluorescence was detectable at the level of individual cells, and isolated colonies. Although oxygen is required for GFP to fluoresce, colonies or cells grown anaerobically were able to fluoresce upon subsequent exposure to air. A combination of selective plating and colony fluorescence allowed a gfp marked strain of E. faecalis to be enumerated after addition to an anaerobic continuous culture of human faecal flora.
Databáze:	OpenAIRE
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