| Autor: |
Banu Sancak, Esvet Mutlu, Osman Sezer Cirit, Tanil Kocagöz, Özge Can, Candan Çicek, Ayça Arzu Sayiner, Özgür Appak, Neval Yurttutan Uyar, Canan Külah, Aysegül Çopur Çiçek, Osman Birol Özgümüs, Yasemin Ay Altintop, Esma Saatçi, Tekin Karsligil, Yasemin Zer, Nevgün Sepin Özen, Yesim Çekin, Zeynep Ceren Karahan, Ebru Evren, Ayse Esra Karakoç, Sultan Gülbahçe, Derya Mutlu, Tugba Bozdemir, Yeliz Tanriverdi Çayci, Canberk Çinar, Meltem Tasbakan, Merve Mert, Ece Çinar, Oya Özlem Eren Kutsoylu, Sesin Kocagöz, Ayse Ertürk, Ilhami Çelik, Ayse Özlem Mete, Müge Günalp Eneyli, Irem Akdemir, Taliha Karakök, Dilara Inan, Aynur Atilla |
| Rok vydání: |
2022 |
| DOI: |
10.21203/rs.3.rs-1298909/v1 |
| Popis: |
Diagnosis of COVID19 is based on reverse transcription-polymerase chain reaction (RT-PCR) testing of nasopharyngeal swab (NPS) samples. As NPS sampling is a discomforting invasive procedure, studies were conducted to investigate the performance of saliva and mouthwash (MW) samples in RT-PCR. There is limited data about MW samples. We have developed a new method for concentrating gargle and mouthwash (GMW) samples to be used in RT-PCR. In our study, we aimed to investigate the performance of concentration of GMW samples in detection of SARS-CoV-2. A paired sample of NPS and GMW samples were collected from patients in 11 centers in Turkey. MW samples were concentrated using MyMagiCon-RW100® (Bio-T, Istanbul, Turkey). NPS, GMW and concentrated GMW samples were tested by RT-PCR for the presence of SARS-CoV2 and the results were compared. The viral RNA was detected in 47.5% of NPS samples, in 28.8% of GMW before concentration and in 37.5% GMW samples after concentration. Concentration of samples increased the number of samples in which SARS-CoV2 RNA was detected by 16.6%. The RT-PCR of concentrated GMW samples yielded better results than the RT-PCR of NPS samples in two centers. Concentrated GMW sampling can be an alternative method to NPS sampling in rapid and accurate diagnosis of COVID-19. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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