Proteomic Profiling of the Secretome of Trichoderma reesei
| Autor: | Siu Kwan Sze, So Fong Cam Ngan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences biology Proteomic Profiling 030302 biochemistry & molecular biology Quantitative proteomics food and beverages Cellulase Biorefinery Proteomics biology.organism_classification 03 medical and health sciences Secretory protein Enzyme chemistry Biochemistry biology.protein Trichoderma reesei 030304 developmental biology |
| Zdroj: | Trichoderma reesei ISBN: 9781071610473 |
| DOI: | 10.1007/978-1-0716-1048-0_17 |
| Popis: | Trichoderma reesei (T. reesei) is the workhorse for the production of industrial cellulolytic enzyme cocktails for cellulose hydrolysis. However, the current industrial process using enzyme cocktails is not efficient enough for the cost-effective generation of cellulosic sugar. Here, we describe a protocol for the application of a state-of-the-art LC-MS/MS-based proteomics method for studying the T. reesei secretome. A protein-free minimal chemically defined cell culture medium must be used for a successful secretome analysis. A lignocellulose substrate can be added to this minimal medium to stimulate the fungal secretion of enzymes specific to that substrate. The secretory proteins in the conditioned medium can be purified for quantitative proteomics profiling. T. reesei secretes several hundred enzymes including cellulases, hemicellulases, pectinases, proteases, oxidoreductases, and many putative proteins when it is stimulated with lignocellulose. By combining an understanding of the basic biomass hydrolytic mechanisms with the discovery of novel enzymes, more effective enzyme cocktails can be designed for a sustainable biochemical-based biorefinery. |
| Databáze: | OpenAIRE |
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