| Autor: |
Takanobu Kato, Yasuhiro Itsui, Sei Kakinuma, Mamoru Watanabe, Yuki Nishimura-Sakurai, Mina Nakagawa, Seishin Azuma, Tetsuya Nakamura, Takaji Wakita, Naoya Sakamoto, Machi Yamamoto, Kiichiro Tsuchiya |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Hepatology Research. 41:258-269 |
| ISSN: |
1386-6346 |
| DOI: |
10.1111/j.1872-034x.2010.00771.x |
| Popis: |
Aim: Studies of the complete hepatitis C virus (HCV) life cycle have become possible with the development of a HCV-JFH1 cell culture system. Methods: In this study, we constructed two fluorescence protein-tagged recombinant JFH1 virus clones, JFH1-EYFP and JFH1-AsRed, as well as two corresponding clones with adaptive mutations, JFH1-EYFP mutant and JFH1-AsRed mutant, that and were as effective as JFH1 in producing infectious virus particles, and investigated their viral infection life cycles. Results: After infection of the fluorescence-tagged mutant viruses, infected cells increased exponentially. In cells, EYFP or AsRed and NS5A were expressed as a fusion protein and co-localized in core proteins. The rate of the cell–cell spread was dependent on the cell densities with a maximum of 102.5/day. Treatment of cells with interferon or a protease inhibitor suppressed expansion of virus-positive cells. Conclusion: Taken together, these results indicate that fluorescence-tagged HCV is a useful tool to study virus infection life cycles and to assist in the search for novel antiviral compounds. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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