In vivo transgenic expression of collybistin in neurons of the rat cerebral cortex

Autor: Tzu-Ting Chiou, Joseph J. LoTurco, John Bear, Angel L. De Blas, Sean Dinallo, Christopher D. Fekete, Celia P. Miralles, Christopher G. Fiondella, Roman Goz
Rok vydání: 2016
Předmět:
Zdroj: Journal of Comparative Neurology. 525:1291-1311
ISSN: 0021-9967
DOI: 10.1002/cne.24137
Popis: Collybistin (CB) is a guanine nucleotide exchange factor selectively localized to γ-aminobutyric acid (GABA)ergic and glycinergic postsynapses. Active CB interacts with gephyrin, inducing the submembranous clustering and the postsynaptic accumulation of gephyrin, which is a scaffold protein that recruits GABAA receptors (GABAA Rs) at the postsynapse. CB is expressed with or without a src homology 3 (SH3) domain. We have previously reported the effects on GABAergic synapses of the acute overexpression of CBSH3- or CBSH3+ in cultured hippocampal (HP) neurons. In the present communication, we are studying the effects on GABAergic synapses after chronic in vivo transgenic expression of CB2SH3- or CB2SH3+ in neurons of the adult rat cerebral cortex. The embryonic precursors of these cortical neurons were in utero electroporated with CBSH3- or CBSH3+ DNAs, migrated to the appropriate cortical layer, and became integrated in cortical circuits. The results show that: 1) the strength of inhibitory synapses in vivo can be enhanced by increasing the expression of CB in neurons; and 2) there are significant differences in the results between in vivo and in culture studies. J. Comp. Neurol. 525:1291-1311, 2017. © 2016 Wiley Periodicals, Inc.
Databáze: OpenAIRE
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