Combined scanning X-ray diffraction and holographic imaging of cardiomyocytes
| Autor: | Martin Krenkel, Stefan Luther, Marten Bernhardt, Claudia Richter, Jan-David Nicolas, Tim Salditt |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physics Electron density business.industry Scattering Small-angle X-ray scattering Momentum transfer Holography 01 natural sciences General Biochemistry Genetics and Molecular Biology law.invention 03 medical and health sciences Reciprocal lattice 030104 developmental biology Optics law 0103 physical sciences Spatial frequency 010306 general physics business Structure factor |
| Zdroj: | Journal of Applied Crystallography. 50:612-620 |
| ISSN: | 1600-5767 |
| DOI: | 10.1107/s1600576717003351 |
| Popis: | This article presents scanning small-angle X-ray scattering (SAXS) experiments on the actomyosin assemblies in freeze-dried neo-natal rat cardiac muscle cells. By scanning the cells through a sub-micrometre focused beam, the local structure and filament orientation can be probed and quantified. To this end, SAXS data were recorded and analyzed directly in reciprocal space to generate maps of different structural parameters (scanning SAXS). The scanning SAXS experiments were complemented by full-field holographic imaging of the projected electron density, following a slight rearrangement of the instrumental setup. It is shown that X-ray holography is ideally suited to complete missing scattering data at low momentum transfer in the structure factor, extending the covered range of spatial frequencies by two orders of magnitude. Regions of interest for scanning can be easily selected on the basis of the electron density maps. Finally, the combination of scanning SAXS and holography allows for a direct verification of possible radiation-induced structural changes in the cell. |
| Databáze: | OpenAIRE |
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