End group analysis of naturally terminated and UV lesion terminated T7 in vitro RNA

Autor:	Robert Millette, Rashid Ali, Walter Sauerbier
Rok vydání:	1976
Předmět:	biology RNA RNA-dependent RNA polymerase Nuclease protection assay Cytidine Molecular biology chemistry.chemical_compound chemistry Biochemistry RNA polymerase Genetics biology.protein Nucleoside Polymerase DNA
Zdroj:	Nucleic Acids Research. 3:3359-3368
ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/3.12.3359
Popis:	The 3' terminal nucleosides of RNA transcribed in vitro by E. coli RNA polymerase from T7 DNA and UV irradiated TN DNA were determined. The 3' terminal nucleoside of naturally terminated (t1 termination site) RNA cytidine. In the case of RNA terminated at UV lesions, it is cytidine in 0 per cent of the molecules and adenosine in the remaining 30 per cent. Cytidine trialcohols are labile in high concentrations of KOH and at high temperature and appear to convert to uridine.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d41305ca19a4667bbac3d08687659a47 https://doi.org/10.1093/nar/3.12.3359 Zobrazit plný text záznamu