Quantitative Reverse Transcription-Polymerase Chain Reaction of Circulating Thyroglobulin Messenger Ribonucleic Acid for Monitoring Patients with Thyroid Carcinoma1
| Autor: | Carole A. Spencer, R. Michael Tuttle, Leonard Wartofsky, Matthew D. Ringel, Pina L. Balducci-Silano, Gary L. Francis, Yvonne H. Sparling, Paul W. Ladenson, Jeffery S. Anderson, Michael A. Levine, Jill L. Silverman, Kenneth D. Burman |
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| Rok vydání: | 1999 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.diagnostic_test business.industry Endocrinology Diabetes and Metabolism medicine.medical_treatment Biochemistry (medical) Clinical Biochemistry Thyroid Thyroidectomy medicine.disease Biochemistry Reverse transcription polymerase chain reaction Thyroid carcinoma Endocrinology medicine.anatomical_structure Immunoassay Internal medicine Carcinoma Medicine Thyroglobulin business Thyroid cancer |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 84:4037-4042 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.84.11.6164 |
| Popis: | Patients with thyroid cancer are monitored for disease recurrence by measurement of serum thyroglobulin (Tg) and iodine-131 (131I) scanning. To enhance sensitivity and to circumvent antibodies that interfere with Tg immunoassays, we have developed RT-PCR assays that detect circulating thyroid messenger RNA (mRNA) transcripts. We now report results using a sensitive quantitative Tg mRNA assay (Taqman; ABI, Foster City, CA) in comparison with immunoassay in patients previously treated for thyroid cancer. We evaluated 107 patients: 84 during T4 therapy, 14 after T4 withdrawal, and 9 at both time points. All patients had near-total thyroidectomy, and 92% received postoperative 131I. Serum TSH, Tg protein, and Tg mRNA were measured. Patients were grouped based on most recent 131I scan or pathologically confirmed disease as having no detectable thyroid tissue (n = 33), thyroid bed uptake (n = 37), cervical/regional adenopathy (n = 21), or distant metastases (n = 16). During T4 therapy, median (range) Tg mRNA va... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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