Expression of the Electrogenic Na+-HCO3--Cotransporter NBCe1 in Tumoral Insulin-Producing BRIN-BD11 Cells
| Autor: | Renaud Beauwens, Christine Delporte, Muhammed S. Soyfoo, Jason Perret, Nurdan Bulur, Myrna Virreira, Karim Louchami, Willy Malaisse, Abdullah Sener |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
geography geography.geographical_feature_category medicine.diagnostic_test Physiology Catabolism Insulin medicine.medical_treatment Immunocytochemistry Biology Islet Molecular biology Endocrinology Western blot Cell culture Internal medicine medicine Tenidap Cotransporter medicine.drug |
| Zdroj: | Cellular Physiology and Biochemistry. 24:187-192 |
| ISSN: | 1421-9778 1015-8987 |
| DOI: | 10.1159/000233257 |
| Popis: | Background/aims: The expression of the electrogenic Na + -HCO 3 - -cotransporter NBCe1 was recently documented in rat pancreatic islet B-cells, it being speculated that such a protein participates in the extrusion of bicarbonate generated by the oxidative catabolism of nutrients from insulin-producing cells. Considering the prevalence of a Crabtree effect in tumoral insulin-producing cells, the possible presence of NBCe1 was now investigated in BRIN-BD11 cells, an insulin-producing cell line established by electrofusion of normal pancreatic B-cells with immortalized RINm5F cells. Methods: The possible presence of NBCe1 in BRIN-BD11 cells was investigated by RT-PCR, western blot analysis and immunocytochemistry. The release of insulin and net uptake of 22 Na + were also measured in the BRIN-BD11 cells. Results: RT-PCR, western blot analysis and immunocytochemistry documented the presence of NBCe1 in BRIN-BD11 cells. A reported inhibitor of NBCe1, i.e. tenidap, (50-100 µM), inhibited basal and hypotonicity-induced insulin release from the BRIN-BD11 |
| Databáze: | OpenAIRE |
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