NIR-II fluorescence in vivo confocal microscopy with aggregation-induced emission dots
| Autor: | Wen Liu, Hequn Zhang, Xiaoming Yu, Bing Guo, Dingwei Xue, Jun Qian, Xianhe Sun, Jing Zhou, Liang Zhu, Wenbin Yu |
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| Rok vydání: | 2019 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Multidisciplinary Materials science business.industry Confocal Resolution (electron density) technology industry and agriculture equipment and supplies 010502 geochemistry & geophysics Laser 01 natural sciences Fluorescence Photon counting law.invention law Confocal microscopy Femtosecond Optoelectronics business 0105 earth and related environmental sciences |
| Zdroj: | Science Bulletin. 64:410-416 |
| ISSN: | 2095-9273 |
| Popis: | Significantly reduced tissue scattering of fluorescence signals in the second near-infrared (NIR-II, 1,000-1,700 nm) spectral region offers opportunities for large-depth in vivo bioimaging. Nowadays, most reported works concerning NIR-II fluorescence in vivo bioimaging are realized by wide-field illumination and 2D-arrayed detection (e.g., via InGaAs camera), which has high temporal resolution but limited spatial resolution due to out-of-focus signals. Combining NIR-II fluorescence imaging with confocal microscopy is a good approach to achieve high-spatial resolution visualization of biosamples even at deep tissues. In this presented work, a NIR-II fluorescence confocal microscopic system was setup. By using a kind of aggregation-induced emission (AIE) dots as NIR-II fluorescent probes, 800 μm-deep 3D in vivo cerebrovascular imaging of a mouse was obtained, and the spatial resolution at 700 μm depth could reach 8.78 μm. Moreover, the time-correlated single photon counting (TCSPC) technique and femtosecond laser excitation were introduced into NIR-II fluorescence confocal microscopy, and in vivo confocal NIR-II fluorescence lifetime microscopic imaging (FLIM) of mouse cerebral vasculature was successfully realized. |
| Databáze: | OpenAIRE |
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