Plating Isolation of Various Catalase-Negative Microorganisms from Soil

Autor: D. P. Labeda, Christina M. Hunt, L. E. Casida
Rok vydání: 1974
Předmět:
Zdroj: Applied Microbiology. 27:432-434
ISSN: 0003-6919
Popis: A unique plating procedure was developed that allows isolation, but not enumeration, of representatives of the catalase-negative soil microflora. The numbers recovered, however, are low as compared to the numbers recovered when the modified dilution-to-extinction isolation procedure is used. The latter procedure provides prolonged inoculation in sealed tubes containing a nutritionally rich broth medium over small submerged agar slants. In contrast, the plating procedure utilizes nutritionally minimal media and the shorter incubations mandated by the inherent problems associated with plating.
Databáze: OpenAIRE