In Vitro Reconstitution of Human Mitochondrial Transcription
| Autor: | Azadeh Sarfallah, Dmitry Temiakov |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Scaffold biology Chemistry TFAM In vitro Cell biology 03 medical and health sciences 0302 clinical medicine Transcription (biology) Mitochondrial transcription Transcription preinitiation complex biology.protein Structural motif 030217 neurology & neurosurgery Polymerase 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071608333 |
| DOI: | 10.1007/978-1-0716-0834-0_3 |
| Popis: | In vitro assay based on a reconstituted mitochondrial transcription system serves as a method of choice to probe the functional importance of proteins and their structural motifs. Here we describe protocols for transcription assays designed to probe activity of the human mitochondrial RNA polymerase and the transcription initiation complex using RNA-DNA scaffold and synthetic promoter templates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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